PCR vs “PCAren’t”

Tolerance of droplet-digital PCR vs real-time quantitative PCR to inhibitory substances.

Employment or Leadership: C.R.L. Teo, National University of Singapore; S.S. Chong, National University of Singapore. Consultant or Advisory Role: None declared. Stock Ownership: None declared. Honoraria: None declared. Research Funding: C.G. Lee, National Medical Research Council; S.S. Chong, National Medical Research Council. Expert Testimony: None declared. Patents: C.R.L. Teo, patent number...

Detection of Human Influenza Viruses in Nasopharyngeal Samples by RT-PCR vs Tissue Culture

Background and Aims: Influenza virus is a major pathogen involved in respiratory illnesses during winter seasons. A variety of diagnostic methods have been developed to identify influenza viruses in clinical specimen. Methods: Nasal and pharyngeal samples taken from patients were inoculated into Madin-Darby canine kidney (MOCK) cells and embryonated chicken eggs (ECEs). The culture media was a...

PCR vs "PCAren't": limitations of PCR-based assays performed on formalin-fixed, paraffin-embedded mucosal biopsy specimens.

Clostridium difficile remains the most common nosocomial cause of diarrhea and is becoming an increasingly important community-acquired infection.1,2 Antibiotic resistance, as well as the recently identified BI/NAP1/027 strain, is responsible for more cases of severe C difficile–associated diarrhea worldwide.3 The diagnosis of C difficile infection is usually based on a combination of history o...

997Saliva vs Urine PCR: The Ideal Sample for congenital CMV Screening and Diagnosis

and Diagnosis Swetha G. Pinninti, MD; Shannon Ross, MD; Zdenek Novak, MD; April Palmer, MD; Amina Ahmed, MD; Pablo J. Sanchez, MD, FIDSA; Marian Michaels, MD; David Bernstein, MD; Kristina N. Feja, MD, MPH; Audra Stewart, DO, MPH; Karen Fowler, DrPH; Suresh Boppana, MD, FIDSA; CMV and Hearing Multicenter Screening (CHIMES) Study; Pediatrics University of Alabama at Birmingham, Birmingham, AL; P...

Performance of TEM-PCR vs. Culture for Bacterial Identification in Pediatric Musculoskeletal Infections